泌乳素对系统性红斑狼疮患者外周血单个核细胞表面CD40/CD154表达的影响

Effect of prolactin on CD40/CD154 expression on peripheral blood mononuclear cells in patients with systemic lupus erythematosus

目的研究泌乳素(PRL)对系统性红斑狼疮(SLE)患者外周血单个核细胞表面CD40、CD154表达的影响,从而探讨其在系统性红斑狼疮发病机制中的作用。方法检测30例系统性红斑狼疮患者和20例正常健康人血清PRL水平,分离外周血单个核细胞(PBMC),采用流式细胞仪检测CD40、CD154的表达水平。结果①高PRL的SLE患者组CD154的表达水平明显高于正常PRL的SLE患者组和正常对照组(P<0.05);②正常PRL的SLE患者组PBMC经重组泌乳素(rhPRL)刺激后CD154的表达水平较刺激前明显增加(P<0.05),而正常对照组PBMC经rhPRL刺激后CD154表达增加不明显(P>0.05);③高PRL的SLE患者组、正常PRL的SLE患者组(加rhPRL)加入溴隐亭(Brc)前后,CD154表达水平无明显差异(P>0.05)。④SLE患者组PBMC表面CD40表达水平与正常对照组差异无显著性(P>0.05)。结论PRL可通过上调PBMC表面CD154的表达,在SLE的发病中起重要作用;Brc对内源性、外源性PRL均无明显抑制作用。

Objective To investigate the effect of prolactin on CD40 and CD154 expressions on the surface of peripheral blood mononuclear cells in patients with systemic lupus erythematosus （SLE） and explore the role of prolactin in the pathogenesis of SLE. Methods The serum prolectin level was detected in 30 SLE patients and 20 healthy volunteers, from whom peripheral blood mononuclear cells （PBMCs） were also isolated to examine the expressions of CD40 and CD 154 using flow cytometry. Results CD154 significantly increased on the PBMCs in SLE patients with high serum prolectin level in comparison with that in patients with normal prolactin level or the normal controls （P〈0.05）. When the PBMCs were incubated with recombinant human prolactin, CD154 expression was significantly increased in SLE patients with normal serum prolactin level （P〈0.05）, but not in the normal control group （P〉0.05）. Incubation of the PBMCs in the presence of bromocriptine did not result in significantly decreased CD 154 expression in SLE patients irrespective of the prolactin level, nor was significant difference found in CD40 expression on the surface of PBMCs between SLE patients and the normal controls（P〉0.05）. Conclusion Prolactin plays an important role in the pathogenesis of SLE by increasing CD 154 expression on the PBMCs, and bromocriptine produces no significant inhibitory effect on either endogenous or exogenous prolectin.