动脉粥样硬化病变中p53抑癌基因结构突变的研究

The studies of the mutations of p53 anti oncogene in Chinese adultsatherosclerosis

本文应用高灵敏、高特异的ＰＣＲ－ＳＳＣＰ方法，首次系统地研究了中国成人ＡＳ病变组织中ｐ５３ａｎｔｉ－ｏｎｃｏｇｅｎｅ的突变。结果发现：受检的８９例ＡＳ组织中的９例有ｐ５３基因的突变（有ＰＣＲ－ＳＳＣＰ的异常），其中６例经测序确定了ｐ５３基因突变的具体位点。结论：在人类的ＡＳ病变中确有ｐ５３ａｎｔｉ－ｏｎｃｏｇｅｎｅ的突变，此可能为ＡＳ形成过程中新的主要机制之一。

Controlling by Chinese healthy adultsnormal artery cells and healthy adults normal blood lymphocytes(DNA), the mutations of p53 anti oncogene in 89 cases of Chinese adults atherosclerotic(AS) plaques were studied detailedly and systematically. The results showed that totally 9 cases(9/89) mutations of p53 gene were found by radiative PCR SSCP, a highest sensitive and highest specific method for the detection of DNA mutations, in which, 6 cases had been further verified by sequencing. In conclusion, from our studies, its clear that there are definite mutations of p53 anti oncogene in some Chinese adults AS plaque cells, and this phenomenon may be a new mechanism in the pathogenesis of human AS damages.