摘要
用RAPD技术对南阳牛种质资源的DNA进行检测,采用Popgene软件对扩增结果进行统计分析表明:筛选的16个引物共得到了236条扩增DNA片断,其中89.7%的扩增条带表现出多态性。平均每个位点有1.989 6个等位基因,有效等位基因数为1.233 3。Nei遗传多样性指数为0.159 7,Shannon's遗传多样性指数为0.270 3。种群总的遗传多样性Ht为0.163 7,种群内的遗传多样性Hs为0.130 5,种群的基因分化系数为0.202 8,种群间的基因流为1.965 3。
The genetic diversity of Nanyang cattle accessions was tested by RAPD technology and the Popgene software. The result showed that a total of 236 bands were amplified by 16 mer arbitrary primers,of which 89.7% bands were polymophic. In average, 1. 9896 alleles was found in each locus,of which the number of effective allele is 1. 233 3. The index of genetic diversity for Nei and Shannon＇s was 0. 159 7 and 0. 270 3, respectively. Ht was 0. 163 7 and Hs 0. 130 5. The gene differentiation coefficient in populations is 0. 202 8 and gene class between populations is 1. 965 3.
出处
《河南农业科学》
CSCD
北大核心
2007年第9期99-100,103,共3页
Journal of Henan Agricultural Sciences
基金
河南省自然科学基金项目(0411034200)
