摘要
目的:探讨RASSF1抑癌基因二种不同转录本在胃癌中的表达情况及与临床的关系。方法:采用RT-PCR方法检测32例胃癌组织及相应的癌旁正常组织,以及51例胃良性病变中RASSF1A、RASSF1B mRNA的表达情况。结果:RASSF1A、RASSF1B在癌旁正常组织中表达缺失率为0,在胃良性病变中有表达缺失,在胃癌组织中的表达缺失率分别为59.4%(19/32)和28.1%(9/32)。RASSF1A、RASSF1B表达与胃癌分化程度及组织学类型无显著相关性。结论:RASSF1二种不同转录本RASSF1A、RASSF1B在癌旁正常组织中可完全表达,在胃癌中的表达缺失率不同,与胃癌的分化程度无明显相关。RASSF1A在胃癌中表达缺失明显,表明RASSF1A是一种具有抑癌作用的基因。
Objective：To investigation the expression lose rate of two kind different RASSF1 transcripts RASSF1 A, RASSF1B in gastric carcinoma and benign gastric disease and discuss their relations with clinical significance. Methods ：The mRNA expression of RASSF1A and RASSF1B were detected by RT-PCR in 32 patients with gastric carcinoma, 32 adjacent non-carcinomatous gastric tissues and 51 case with benign gastric disease. Results.The rate of expression loss of transcripts A and B in adgastric carcinoma were 59. 4% and 28.1% , respectively. The rate of expression loss of transcripts A and B in adjacent non-carcinomatous were zero. RASSF1A and RASSF1 B mRNA expression have no statistical relation with different grade of gastric carcinoma. Conclusion：The two different RASSF 1 transcripts RASSFl A, RASSF1B are all expression in adjacent non-carcinomatous. RASSFl A is a significant expression loss in gastric carcinoma, and has no statistical relation with different grade of gastric carcinoma. The result indicates that RASSF1A is a suppressor gene.
出处
《临床肿瘤学杂志》
CAS
2007年第9期663-665,共3页
Chinese Clinical Oncology
