冠心Ⅰ号治疗冠心病的临床和实验研究

A Clinical and Experimental Study on the Use of Guanxin No.1 in the Treatment of Coronary Heart Disease

目的:研究冠心Ⅰ号的临床疗效和作用机制。方法:应用具有益气养阴、活血通阳功效的冠心Ⅰ号对20例冠心病患者进行临床治疗观察,并用冠心Ⅰ号对具有动脉粥样硬化动物模型的大鼠灌胃,观察其对主动脉形态及对主动脉MMP-9蛋白、MMP-9/TIMP-1蛋白比值表达的影响。结果:冠心Ⅰ号治疗心绞痛总有效率为90%;临床中医证候总疗效总有效率为95%;心电图总有效率为45%。动物实验表明:冠心Ⅰ号可改善动脉粥样硬化动物模型大鼠的血管内膜细胞增生,降低MMP-9的蛋白表达,调节MMP-9/TIMP-1失衡状态。结论:冠心Ⅰ号是通过降低MMP-9的蛋白表达,调节MMP-9/TIMP-1失衡状态,改善血管内膜细胞增生及心肌缺血,减轻动脉粥样硬化病变程度。

Objectives： To study the clinical effects and therapeutic mechanisms of the application of Chinese traditional medicine,the Guanxin No. 1 in the treatment of coronary heart disease. Methods ：The clinical effects were observed in 20 patients with coronary heart disease, to whom Guanxin No. 1 were given. The rats of atherosclerotic model were used as experimental models with cardiovascular disease. The rats were fed with Guanxin No. 1 and the effects on the aorta shapes and expressions of MMP -9 protein and MMP -9/TIMP - 1 of rats were noticed. Results ： The total effective rate of angina, clinical characteristics of syndromes of TCM and ECG respectively was 90% ,95% and 45% with Guanxin No. 1. Experimental study showed that Guanxin No. 1 could improve arterial smooth muscular cells cultured, reduce expressions of MMP - 9 protein and adjust the state of MMP - 9/TIMP - 1 imbalance from the rats of atherosclerotic model. Conclusions ： Guanxin No. 1 could improve arterial smooth muscular cells cultured, myocardial ischemia and reduce the severity of coronary atherosclerosis by reducing expressions of MMP - 9 protein and adjusting the state of MMP - 9/TIMP - 1 imbalance.