选择性COX-2抑制剂NS398对前列腺癌PC-3细胞增殖与凋亡的影响

Effect of selective cyclooxygenase-2 inhibitor NS398 on the proliferation and apoptosis of prostate cancer cell line PC-3 in vitro

目的观察环氧化酶-2(COX-2)抑制剂NS398对前列腺癌细胞PC-3增殖和凋亡的影响。方法采用四甲基偶氮唑蓝法(MTT法)检测不同浓度和不同时间NS398对PC-3细胞增殖的影响;RT-PCR法检测不同浓度NS398作用PC-3细胞24h后COX-2 mRNA的表达;酶联免疫测定法(ELISA)检测不同浓度NS398作用PC-3细胞24h后PGE2释放水平;流式细胞仪检测不同浓度NS398作用PC-3细胞24h后细胞凋亡情况。结果NS398可以抑制PC-3细胞的增殖,呈时间和剂量依赖性;RT-PCR和ELISA法检测结果显示,随着NS398浓度增高,PC-3细胞COX-2 mRNA表达和PGE2释放水平呈下调趋势;细胞凋亡检测结果显示100、200μmol/LNS398对PC-3细胞具有诱导凋亡的作用。结论NS398可能通过COX-2依赖性途径抑制前列腺癌PC-3细胞增殖,促进肿瘤细胞凋亡。

Objective To investigate the effect of cyclooxygenase-2 inhibitor NS398 on the proliferation and apoptosis of prostate cancer cell line PC-3 in vitro. Methods The proliferative inhibition of PC-3 cells was observed by methyl thiazol tetrazolium（MTT） rapid photocolorimetric assay; the expression of COX-2 mRNA in cultured PC-3 cells was examined by RT- PCR; the release levels of PGEz was measured by enzyme-linked immunosorbent assay（ELISA） ; PC-3 cells cultured in different concentrations of NS398 medium for 24 h was marked with Annexin V-FITC and PI for the cell apoptosis assay by flow cytometry. Results After the PC-3 cells were treated with different concentrations of NS398, the inhibitory rate on proliferation increased with the increasing of concentration and days. The expression of COX-2 mRNA and the release levels of PGF2 were decreased compared with those of the control group. PC-3 cells cultured in 100 and 200 μmol/L NS398 medium for 24 h had significantly higher incidence of apoptosis. Conclusion NS398 can inhibit proliferation of human prostate cancer cell line PC-3 and induce its apoptosis in vitro, which may contributed to the COX-2 dependent pathway.