摘要
目的研究耐甲氧西林金黄色葡萄球菌（MRSA）的基因多态性分型特征，为控制感染提供分子流行病学依据。方法利用一条10bp随机引物，建立随机引物多态性（RAPD）分析方法，并利用这一方法对44株MRSA进行基因分型的研究。结果44株MRSA中有30株产生RAPD指纹图谱，经电泳得到2．8条片段，可分为5个型，其中Ⅲ型菌株占50％。结论通过RAPD分型研究，可了解MRSA的基因型流行特征，为控制感染提供分子流行病学依据。
Objective To conduct the study on the analysis of genetic polymorphic of Methicillin - resistant Staphylococcus （MRSA） and provide molecular epidemiological evidence for controlling MRSA infection. Methods By using short single oligomer primer of 10 bases sequences in polymerase chain reaction, randomly amplified polymorphic DNA（RAPD） was used to analyze MRSA which were previously isolated from the samples of infected patients in the 1st Hospital of Sun Yat - sen University. Results There 44 stains of MRSA produced 30 fingerprints, which could be observed in agarose gel and classified into 8 genotypes. Among which type [U occupied 50%. Conclusion RAPD results reveals the special epidemic genotypes of MRSA in the 1^st Hospital of Sun Yat - sen University and this study can provide molecular epidemiological evidence for control of MRSA infection.
出处
《中国热带医学》
CAS
2007年第10期1753-1754,1762,共3页
China Tropical Medicine
