摘要
目的:研究人脐血间充质干细胞(MSCs)对异体外周血T淋巴细胞抑制的影响.方法:分离、扩增脐血MSCs,流式细胞术检测表面标志以鉴定.取分离培养的脐血MSCs,与经分离的异体淋巴细胞共培养,在植物血凝素(PHA)刺激下,用MTT还原法测定细胞增殖,观察脐血MSCs对PHA刺激的T淋巴细胞转化抑制作用.采用ELISA检测培养上清中IFN-γ和IL-4的分泌水平.结果:脐血MSCs在PHA刺激下可抑制T淋巴细胞增殖,且其培养上清也具有抑制异体淋巴细胞增殖转化能力.ELISA检测7d共培养上清中IFN-γ和IL-4两种细胞因子的含量,发现IFN-γ分泌水平下调、IL-4分泌水平部分上调.结论:脐血MSCs具有抑制异体淋巴细胞免疫反应,降低移植物抗宿主反应(GVHD)的作用.具有研究价值和应用潜能.
AIM： To study the effects of human umbilical cord blood-derived mesenchymal stem cells （MSCs） on peripheral blood T lymphocytes. METHODS： MSCs were isolated from normal human umbilical cord blood, cultured in vitro, purified and then identified by examining surface marker with FCM. Lymphocytes were isolated from allogenic peripheral blood and identified with FCM. The MSCs and lymphocytes were co-cultured in 96-well plates for 72 h. T lymphocyte proliferation was measured by using MTT reduction method and the effect of MSCs on the T lymphocyte transformation stimulated by PHA was observed. On day 7 of co-culture, the culture supernatant was collected to detect the expressions of IFN-γ and IL-4 with ELISA. RESULTS： Human umbilical cord blood-derived MSCs inhibited T lymphocyte proliferation and PHA- induced lymphocyte transformation. The secretion of IFN-γ in supernatant was significantly down-regulated, while the expression of IL-4 was partially increased. CONCLUSION： Human umbilical cord blood-derived MSCs can inhibit the immune response of allogenic T lymphocytes and alleviate the grafe-versus-host diseases.
出处
《第四军医大学学报》
北大核心
2007年第18期1649-1651,共3页
Journal of the Fourth Military Medical University
基金
兰州军区医药卫生科研基金(LXH-2005013)
