十五个微卫星标记在超细型细毛羊上的遗传多样性的研究

Study on Genetic Diversity of Fifteen Microsatellite Markers in Superfine Merino Sheep

本研究选取了绵羊1号和3号染色体上的十五个微卫星位点,对超细型细毛羊的六个父系半同胞的232个后代的遗传多样性进行了检测。计算了各位点的等位基因频率(P)、杂合度(H)、多态信息含量(PIC)和有效等位基因数(N)。结果表明:十五个微卫星位点中有七个未检测到多态,另外八个在超细型细毛羊中表现出较好的多态性。在这八个多态性标记中,微卫星MAF64位点的多态信息含量最高(PIC=0.8399),微卫星BL-4位点的多态信息含量最低(PIC=0.4201)。多态性标记在群体中的平均等位基因数为5.6个,平均多态信息含量PIC=0.64,平均杂合度H=0.67,平均有效等位基因数N=3.7。经分析,超细型细毛羊群体中的遗传杂合度较高,表明其遗传背景复杂,遗传多样性较为丰富。同时这八个多态性微卫星位点也可用于超细型细毛羊羊毛各性状的进一步研究。

In this study, the genetic diversity of 232 samples in six paternal families of half-sibs of superfine merino sheep were studied, by using fifteen microsatellite markers mapped to sheep chromosome 1 and chromosome 3. Allele frequencies（p）, mean heterozygosity（H ）polymorphisms information content（PIC）, and average effective number of alleles（N ）were calculated. The results showed that 8 of 15 microsatellites loci were remarkable polymorphic, the others were not polymorphic. Among the polymorphic microsatellite markers, polymorphisms information content of Mar64 loci is the highest （PIC=0.8399）, and polymorphisms information content ofBL - 4 loci is the lowest （PIC=0.4201）. In this population, the average PIC is 0.64, H is 0.67, N is 3.7. By analysis, genetic heterozygosity of superfine merino sheep is much high, which showed that genetic background is complex, genetic polymorphism is plentiful in the population. And those 8 polymorphic microsatellite markers can also be applied in further analyzing other characteristics of wool in superfine merino sheep.