摘要
目的建立高效液相色谱测定补骨脂不同有效部位中补骨脂素和异补骨脂素含量的方法。方法高效液相色谱法。Kromasil RP-C18色谱柱(4.6mm×250mm,5μm);流动相:甲醇-水(65∶35);流速:1.0mL·min-1;检测波长:245nm。结果补骨脂素进样量在10.5ng～525ng,异补骨脂素进样量在9ng～450ng内线性关系良好;相关系数:补骨脂素r=0.9993,异补骨脂素r=0.9999。不同有效部位中补骨脂素和异补骨脂素的含量有差别,其中乙酸乙酯部位(C)中含量最高,正丁醇部位(D)和水部位上清液(E)中含量很低。结论该法操作简便,结果准确,重现性好。
Objective To establish a HPLC method for the assay of psoralen and isopsoralen in different effective extracts of Fructus Psoraleae. Methods HPLC was carried out on the column of Kromasil RP-C18. The mobile phase was methanol -water （65 ： 35） . The flow rate was 1.0 mL/min and the UV detection wavelength was 245 nm. Results Good linearity of psoralen was showed within the range of 10.5 ng - 525 ng （ r = 0. 999 3） and isopsoralen within the range of 9 ng - 450 ng （ r = 0. 999 9） . The content of psoralen and isopsoralen differed in different extractions of Fructus Psoraleae. Among them, the extract C （extracted by ethyl acetate ） contained the highest contents of psoralen and isopsoralen, while the contents of psoralen and isopsoralen were very low in the extract D （extracted by n - butyl alcohol） and E （supernatant of water extract）. Conclusion The method is simple, accurate and reproducible. The anti - asthma effect and the dose - effect relationship of the different effective extracts of Fructus Psoralea need further pharmacodynamics study.
出处
《中药新药与临床药理》
CAS
CSCD
2007年第5期380-382,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广州中医药大学总体规划课题(C02045)
