高效液相法测定骨愈灵胶囊中人参皂苷Rg_1的含量

Determination of Ginsenoside Rg_1 in Guyuling Capsule by HPLC

目的建立骨愈灵胶囊中人参皂苷Rg1的含量测定方法。方法采用高效液相色谱法,C18色谱柱(4.6mm×150mm,5μm);流动相:乙腈-0.5%磷酸溶液(23∶77);检测波长:205nm。结果人参皂苷Rg1在1.05μg～7μg之间,峰面积与进样量呈良好的线性关系,r=0.9996。平均回收率为98.80%,RSD=1.57%。结论该方法简单可行,重现性好,可用于骨愈灵胶囊的质量控制。

Objective To establish a HPLC method for the content determination of Ginsenoside Rg1 in Guyuling Capsule. Methods HPLC was performed on a C18 column , the mobile phase consisting of acetonitrile - 0. 5 % H3PO4 （23 ： 77 ）and detection wavelength at 205 nm. Results The μg, r = 0. 999 6. The average recovery was 98.80 % , linearity of Ginsenoside Rg1 was good in the range of 1.05 μg - 7 and RSD was 1.57 %. Conclusion The method is simple, feasible, and reproducible , and can be used for the quality control of Guyuling Capsule.