摘要
目的建立降脂灵胶囊中大黄酚的含量分析方法。方法采用RP-HPLC法,色谱柱:Nova-Pak C18柱(3.9mm×150mm,4μm);流动相:甲醇-0.1%磷酸(85∶15);流速:1mL·min-1;检测波长:254nm,通过不同进样体积,产生不同响应值来制备标准曲线,进而定量。结果该方法在0.0375～0.7500μg范围内线性关系良好(r=0.9999),平均加样回收率为98.31%,RSD为1.36%(n=6)。结论该方法简便、准确、重现性好,可用于降脂灵胶囊的质量控制。
Objective To establish a method for the determination of Chrysophanol in Jiangzhiling Capsules. Methods RP - HPLC was used for samples separating on a Nova - Pak C18 column. The mobile phase consisted of methanol - 0. 1% H3PO4 （85 ： 15） The detection wavelength was 254 nm and the flow rate was 1 mL · min^-1. Results The standard curve of Jiangzhiling Capsules was linear in the rang of 0. 037 5 -0. 750 0 μg （ r = 0. 999 9） with average recovery of 98.31% （RSD = 1.36 % ）. Conclusion The method is simple, accurate and precise. It can be used effectively for the quality control of Jiangzhiling Capsules.
出处
《中药新药与临床药理》
CAS
CSCD
2007年第5期392-394,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
