大豆营养生长期的QTL分析

QTL Analysis of Soybean Vegetative Stage

利用153个株系组成的源自美国半矮秆有限性品种Charleston和亚有限性品种东农594的重组自交系群体。在东北农业大学香坊试验站与东北农业大学校内试验田进行了两年三点的试验。采用国际标准对大豆营养生长期进行划分,对观察株系从破土开始记录,每日跟踪调查,直到群体全部成熟。结果表明生育期在群体中呈正态分布,在不同环境条件下,个体间有差异。采用QTLmaper2.0统计软件对生育期进行QTL定位和上位性分析。共检测到主效应的QTL59个,上位性的QTL61对。涉及到A1、C2、D1a、D1b、B1、I、N、G等七条连锁群,上位性分析也首次揭示出不同位点间的互作关系。揭示了大豆营养生长过程中,其QTL的发育特点,找到了可以在不同环境下稳定存在的QTL位点。

In order to Detect the quantitative trait loci （QTLs） conferring the development of vegetative stage and provide Provide the relative theory to soybean molecular marker assistant breeding. A recombinant inbred line （RIL） population including 153 lines, derived from a cross between soybean cultivars Charleston and Dongnong 594 in two years were used in this experiment. The RIL population was planted in field in Xiangfang Station of Northeast Agricultural University, Harbin, China in 2004 and 2005. And RIL population was also plant in courtyard of Northeast Agricultural University by potted. The growth stage data of tested plants were investigated from the emergence to mature each day, the standard as the international measure. Results showed that the growth data distribute as single hillside profile. Different individual had little different appearance of growth stage in different year. The QTL was analyzed by main effect and epistasis effect in three point environments in two years. The soft ware was QTLmaper2.0. We found 59 QTLs had main effect and 61 pairs QTLs had epistasis effect. A1, C2, D1a, D1b, B1, I, N and G linkages were found have relation with these QTLs. The relation between different QTL loci was detected by epistasis analysis. We analyzed the developmental rule of soybean grow in vegetative stage, found the stable locus that can be detected in different environment.