黄瓜种质资源遗传多样性的ISSR分析

Analysis of Genetic Relationships of Cucumber (Cucumis sativus L.) Germ-plasm by ISSR Markers

利用ISSR技术对46份黄瓜材料分析,结果表明,8个ISSR引物在46份黄瓜种质中共扩增出42条带,多态性带的比例为85.71%。由Jaccard方法计算遗传相似系数可知,这些材料间的遗传相似系数的变化范围是0.3030～0.8750,其中23(ZN1)和42(四川寸金子)的遗传距离最小(0.3030),其遗传距离最远,34(Edipse)与43(杨泾黄瓜)之间的遗传相似系数最高(0.8750),其遗传距离最近。由聚类分析结果表明,若以相似性系数为0.52为阈值时,可将46份黄瓜品种聚为两类,一类以较强的雌性表现为特征,另一类则多表现为普通性型。若以0.54的相似系数为阈值,46份黄瓜材料可以被分为四大类,虽然不能完全按照生态型将现有黄瓜资源完全聚类,但总体而言,来自于同一生态型的多数资源可以聚在不同的类或亚类中。

With 8 ISSR primers, 42 bands were amplified in 46 cultivated accessions of cucumber, of 36 bands （85.71%） were polymorphism. The genetic similarity coefficients of 46 cultivated accessions were ranged from 0.3030 to 0.8750, the coefficient between of the line 23（ZN1）and 42（SichuanCunjinzi）were lowest （0.3030）, which means the longest genetic distance between them. And also the highest coefficient was found between the line 34 （Edipse）and 43（Yangjing）,which shows the shortest genetic distance between them. The culstering of 46 tested accessions showed that cultivars used in the present study are divided into 2 groups at the coefficient threshold of 0.52, One group was characterized with stronger gynoecious phyenotype, and the other for general sex phenotype. If the coefficient threshold was shift to 0.54, 46 tested accessions were culstered four groups. Generally, most of the same ecotypes used in this research could be grouped into same group and subgroup.