拉莫三嗪、托吡酯对癫癎大鼠的脑电图及Bcl-2、Bax表达的影响

The effects of lamotrigine and topiramate on electroencephalogram and the expression of Bcl-2 and Bax of the epilepitic rats

目的:探讨拉莫三嗪、托吡酯对青霉素致大鼠行为和脑电图的影响及其对海马神经元的保护作用。方法:观察青霉素致癎组、拉莫三嗪组、托吡酯组、丙戊酸钠组大鼠癎性发作,记录脑电图。免疫组化SABC染色观察Bcl-2、Bax蛋白的表达。结果:拉莫三嗪组、托吡酯组与丙戊酸钠组脑电癎样放电减少,这3组比较癫癎发作潜伏期和发作持续时间有统计学意义(P<0.01)。这3组较青霉素致癎组Bcl-2细胞增多,Bax细胞减少,托吡酯组、拉莫三嗪组较丙戊酸纳组Bcl-2细胞增多,以上差异均有统计学意义(P<0.01)。结论:托吡酯、拉莫三嗪抗癫癎疗效优于丙戊酸钠,且对青霉素致癎大鼠模型海马神经元有保护作用。

Objective：To explore the effect of lamotrigine（LTG）and topiramate（TPM）on the behaviors and electroencephalogram （EEG）of epileptic rats induced by penicillin,and to explore the protection of LTG and TPM for the neurons of hippocampus. Methods：The seizures were observed in the group of epileptic rats induced by penicillin,the group of the rat given LTG,the group of the rat given TPM,the group of the rat given Sodium Valproate,and EEG was recorded in these groups.Immunohistochemical staining was used to observe the expression of Bcl-2 and Bax.Results：The epileptiform discharges decreased in the group of the rat given LTG and the group of the rat given Sodium Valproate,compared the group of the rat given LTG and TPM with the group of the rat given Sodium Valproate.We found that the latencies and durations of seizure between the group of the rat given LTG and the group of the rat given Sodium Valproate had significant difference（P〈0.01）.Compared with the epileptic group induced by penicillin,Bcl-2 positive cells increased and Bax positive cells decreased in the group of the rat given LTG and the group of the rat given odium Valproate（P〈0.01）.Compared with the group of the rat given Sodium Valproate,Bcl-2 positive cells in the group of the rats given LTG group and in the group of the rats given TPM were increased（P〈0.01）.Conclusion：LTG and TPM was better than the Sodium Valproate in the anti-epileptic effects.LTG and TPM can protect hippocampal neuron in the epileptic rats induced by penicillin.