棉子糖低钾右旋糖酐液对猪供体肺灌注保护作用的病理学研究

A pathologiacal study of pig lung donor perfused with raffinose-low potassium dextran solution for lung transplantation

目的:探讨棉子糖低钾右旋糖酐液(raffinose-low-potassium dextran solution,R-LPD液)供肺灌注保存后对肺组织形态学变化的影响。方法:应用光镜、电镜和免疫组化技术对低钾右旋糖酐液(low-potassium dextran solution,LPD液)和R-LPD液灌注保存的各10例猪供肺组织按不同的保存时间段进行对比观察。结果:随着保存时间的延长,供肺的形态结构改变也逐渐明显,保存24h后,LPD液灌注组肺泡间隔断裂,肺泡上皮细胞变性、坏死、脱落,肺泡壁及血管周围间质组织水肿;而R-LPD液灌注组形态改变则相对较轻。结论:R-LPD液与LPD液相比,在供肺灌注保存中更能起到保护肺泡上皮细胞的作用。

Objective：To observe the change of the morphological structure in lung perfused with raffinose-low-potassium dextran solution（R-LPD＇S）.Methods：The 20 pig donor lung perfused with low-potassium dextran solution（LPD＇S）and R-LPD＇S was observed by light microscope,electronic microscope and immunohistochemistry.Results：Morphologic changes of the structure in the tissue cell were gradually obvious along with time.After 24 h of preservation,the tissue of donor lung perfused with LPD＇S showed a decreased number of intact typeⅡpneumocytes and significant cellular necrosis.Interstitial and alveolar edema with interstitial macrophage infiltration was also observed.Alveolar capillaries were collapsed.In contrast,R-LPD＇S lungs showed only mild alterations such as minor interstitial edematous expansion,fewer damaged cells,and minor capillary injury.Conclusion.R-LPD＇S exerts a cytoprotective effect on preservation of donor lung during pulmonary grafts,and it should be superior to LPD＇S for this purpose.