CD40、PCNA在人膀胱移行细胞癌中的表达及意义

Expression of CD40,PCNA on Human Bladder Transitional Cell Cancer and Their Clinical Significance

【目的】探讨CD40、PCNA在人膀胱移行细胞癌(BTCC)中的表达及临床应用价值。【方法】采用免疫组化技术检测56例BTCC组织、20例正常膀胱组织中的CD40和PCNA的表达,分析CD40、PCNA表达水平与肿瘤病理分级、临床分期相关性,以及两指标间的相关性。【结果】①CD40、PCNA在BTCC中的表达为66.1%、64.3%,明显高于正常膀胱黏膜。②CD40在高分化、表浅性BTCC中的表达为91.7%、83.3%,明显高于中低分化、浸润性BTCC。而PCNA在高分化、表浅性BTCC中的表达为33.3%、52.8%,明显低于中低分化、浸润性BTCC。③CD40、PCNA在BTCC中表达呈负相关。【结论】CD40在BTCC发生过程中可能起重要的作用,可作为诊断、评估恶性程度的指标。

To investigate the expression of CD40, PCNA in bladder transitional cell cancer （BTCC） and normal bladder tissues ,and study their clinical significance. [Methods] Immunohistochemical method was used in detecting the expression of CD40 , PCNA in 56 cases of BTCC and 20 cases of normal bladder tissues. The investigation included..①the relationships of CD40 and PCNA to BTCC in various pathological grades and clinical stages②whether there was correlation between CD40 and PCNA. [Results]③The expression rate of CD40 ,PCNA in BTCC was 66.1% ,64.3% respectively, and there were significant differences compared with that in the normal bladder tissues. ④The expression rate of CD40 in well-differentiated , superficial BTCC was 91.7% ,83.3% respectively, higher than poorly-differentiated ,invasive BTCC. The expression rate of PCNA in well-differentiated, superficial BTCC was 33.3 %, 52.8% respectively, lower than poorly-differentiated ,invasive BTCC. ⑤Significant negative correlation was found between CD40 and PCNA in BTCC. [Conclusion] CD40 may play an important role in the carcinogenesis Of BTCC and may become a useful index to diagnose and evaluate the malignancy of BTCC.