大鼠创伤性休克后白介素-8,内皮素与降钙素基因相关肽水平的变化及意义

Changes of plasma IL-8,ET and calcitonin gene-related peptide on traumatic shock in rats

目的:探讨创伤性休克对大鼠血浆内皮素(ET)、白介素-8(IL-8)、降钙素基因相关肽(CGRP)水平及动脉血气分析的影响。方法:采用后肢创伤法建立创伤性休克大鼠模型,测定创伤前后及复苏后1、3、5、12hET、IL-8、CGRP及动脉血气分析水平。结果:在休克末及复苏后1、3、5、12h,大鼠血浆IL-8、ET、CGRP水平及动脉血二氧化碳分压(PCO2)、氧分压(PO2)及氧饱和度(SO2)发生明显变化。血浆IL-8,ET和CGRP含量与休克前相比较明显升高,峰值出现在复苏后5h,同时伴有PCO2升高,而PO2和SO2明显下降。结论:创伤性休克血浆IL-8,ET、CGRP水平明显升高且伴随着PCO2、PO2和SO2的变化。IL-8,ET和CGRP在创伤性休克中起着重要的作用。

Objective：To observe the effects of traumatic shock on the exchanges of plasma Interleukin 8（IL-8） ,endothelin（ET） ,ealeitonin-gene related peptide（CGRP）and arterial blood gas analysis （ABGA）in rats. Methods：The rats were shocked by fracturing the posterior limbs and the plasma concentration of ET and CGRP and arterial blood gas analysis in the rats were compared before shock and 1, 3, 5 and 12 h after resuscitation. Results：The significant changes were observed in the level of IL-8,ET and CGRP and PCO2 and PO2 and SO2 in blood samples at the end of shock and 1,3,5 and 12 h after resuscitation. The results of IL-8, ET and CGRP increased significantly compared with that of pre-shock. Tbe summit occurred 5 h after resuscitation. The results of PCO2 in ABGA also showed the significant increase, while the results of PO2 and SO2 declined. Conclusion：The traumatic shock affect significantly on the changes of ET and CGRP and ABGA in rats, and ET and CGRP played an important role in traumatic shock resuscitation.