胃癌组织差异蛋白表达谱的初步研究

Preliminary analysis of two-dimensional gel electrophoresis maps in gastric carcinoma

目的：建立胃癌和癌旁胃黏膜组织pH 4-7蛋白质表达谱,分析胃癌和癌旁黏膜组织中蛋白质表达的差异。方法：提取胃癌及癌旁胃黏膜组织总蛋白,采用二维电泳获得胃癌和癌旁胃黏膜组织蛋白质表达谱并进行分析。结果：通过比较分析5对胃癌和癌旁胃黏膜组织pH 4-7蛋白质表达谱,共发现128个差异表达的蛋白质点,其中仅在胃癌组织中表达的蛋白质点56个,胃癌组织中失表达的蛋白质点27个,32个点在胃癌组织中表达上调,13个点在胃癌组织中表达下调。结论：胃癌与癌旁胃黏膜组织之间存在蛋白质表达差异。寻找胃癌与癌旁胃黏膜组织之间表达差异的蛋白质,为阐明胃癌发生的分子机制奠定基础。

Objective： To establish the two-dimensional gel electrophoresis pH 4 -7 maps of human gastric carcinoma and paired paracancerous mucosa, and to analyze differently expressed proteins of human gastric carcinoma and paired paracancerous mucosa. Methods： The total proteins of human gastric carcinoma and paired paracancerous mucosa were extracted, separated and analyzed by two-dimensional electrophoresis （2-DE）. Results：By comparing and analyzing pH 4 -7 protein expression maps of 5 cases of gastric carcinoma and paired paracancerous mucosa, 128 differently expressed protein spots were detected, of which 56 expressed only in gastric carcinoma, 27 in normal gastric mucosa tissues, 32 more highly and 13 more lowly in gastric carcinoma than in paracancerous mucosa tissues. Conclusion ： Proteins express differently in gastric carcinoma from paired paracancerous mucosa. Searching for differently expressed proteins between the two types of tissues may throw light on the molecular mechanism of gastric carcinoma.