分泌抗人促甲状腺激素(hTSH)单克隆抗体杂交瘤细胞株的建立

ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING hTSH McAb

用ＳＰ２／０骨髓瘤细胞与经人促甲状腺激素（ｈＴＳＨ）免疫的ＢＡＬＢ／Ｃ小鼠的脾细胞融合，融合率６０．４％，抗体阳性率４．３％，经３～４次克隆化，筛出３株稳定分泌ＴＳＨＭｃＡｂ的杂交瘤细胞株。用放射免疫分析法（ＲＩＡ）检测小鼠腹水，抗体效价为１０－４时，抗体结合率为４５．５％。用ＥＬＩＳＡ方法检测小鼠腹水，抗体效价达１０－５。其中３株细胞做琼脂糖免疫双扩散试验，均显示为ＩｇＧ１，亚类。染色体计数为９５对，证明为杂交瘤细胞。

SP2/0 myeloma cells are fused with spleen cells of BALB/C mice immunized with hTSH. The rate of fusion is 60.4%,and the positive rate of antibody is 4.3%. After cloning, 3 hybridoma cell lines of secretion hTSH McAb is established. When the activity of McAb of ascites diluted to 1∶10 -4 is tested by RIA, the binding rate is 45.5%.Ig class and subclass are determined with agar double diffusion test, 3 hybridoma cell lines secreting McAb are IgG 1 subclass.