百令胶囊的指纹图谱鉴别研究

Identification of Bailing Capsule by RP-HPLC fingerprint

目的:建立百令胶囊专属性的指纹图谱鉴别方法。方法:以发酵虫草菌粉对照药材(CS-C-Q80)与腺苷、尿苷对照品对照,采用HPLC检测方法,色谱柱:Alltima C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.04 moL/L磷酸二氢钾溶液为流动相,梯度洗脱,流速为0.8 ml/min,测定波长:260 nm。结果:采用本法可以区别其它类似虫草产品。稳定性、精密度、重现性的相对标准偏差(RSD)均小于3%,在相同的色谱条件下,经不同型号的HPLC仪,不同的色谱柱比较,相对保留时间的相对标准偏差小于5%。结论:本法操作简便,专属性强,重现性好,可作为百令胶囊的指纹图谱鉴别方法。

AIM： To establish a specific method for identifying Bailing Capsule （Synnematum sinense, Yin et shen sp. now）. METHODS： Using fermented cordyceps powder（Cs-C-Q80）,adenosine and uridine as reference standards, a HPLC fingerprint at 260 nm has been established for identification of Bailing Capsule, using Alltima C18 （250 mm ×4.6 mm,5μm） column and acetonitrile -0.04 mol/L KH2PO4 in gradient elution as a mobile phase. The flow rate was 0.8 ml/min. RESULTS： The relative standard deviations of stability, precision and reproducibility was less than 3% respectively. The relative standard deviation of relative retention time was less than 5% compared with other HPLC apparatuses and different colunms. CONCLUSION： The method established in this paper has the exclusivity and can be used to identify the Bailing Capsule.