检测色氨酸的免标记生物传感器研制

A Novel L-Tryptophan Sensor Based on Electrochemical Impedance

利用Au-S化学键的作用,在金基底上组装L-半胱氨酸(L-Cys),通过铜离子的配位作用,构建Cu2+/L-Cys/Au自组装膜.考察该自组装膜的组装条件、电化学特征、重现性与稳定性,研究不同浓度的L-色氨酸中膜的识别能力和识别选择性.结果表明,自组装膜具有对L-色氨酸识别的选择性,在不同浓度的L-色氨酸中,膜的电化学阻抗会发生明显变化.因此,利用该膜对L-色氨酸(L-Try)的选择性识别引起电化学阻抗变化的特性,可构建一种新型的免标记电化学阻抗法色氨酸传感器.

With the help of chemical bonding of Au--S, L-cysteiney （L-Cys） was assembled onto the gold surface to form L-Cys/Au self-assembled monolayer. Taking advantage of coordinate action of Cu^2＋ with L-cysteine, the Cu^2＋/L- Cys/Au self-assembled membrane was constructed. The films assembling conditions; electrochemical characteristics, reproducibility and stability are discussed, and the reorganization selectivity for L-tryptophan （L-Try） are also studied. The experimental results showed that the film Cu^2＋/L-Cys/Au could selectively recognize for L-tryptophan. When Cu^2＋/L-Cys/Au acted on L-tryptophan, the electrochemical impedance changed, and the self-assembled membrane could selectively recognize L-tryptophan. A novel L-tryptophan sensor based on electrochemical impedance was built up.