黄芪注射液对肝纤维化大鼠模型肝细胞胶原及TGF-β_1表达的影响

Effect of Astragalus Injection on Expression of Collagen and TGF-β_1 in Rat Model of Experimental Fibrosi

目的探讨黄芪注射液抗肝纤维化及对TGF-β1、Col-Ⅰ表达的影响。方法将35只SD大鼠随机分为正常对照组、CCl4诱导肝纤维化模型组和黄芪干预组。至造模第10周,处死所有大鼠,采用PV免疫组化方法检测Col-Ⅰ在各组肝组织中的表达,ELISA法检测TGF-β1在各组血清中的表达。结果经CCl4诱导成功建立大鼠肝纤维化模型,随着肝纤维化程度的进展,肝组织中Col-Ⅰ和血清中TGF-β1表达明显增强。在正常对照组、黄芪干预组和肝纤维化模型组肝组织中Col-Ⅰ表达的平均光密度值分别为0.298、0.315、0.503,模型组与前两组比较差异均有显著性(P<0.01),而黄芪干预组与正常组肝组织比较差异无统计学意义(P>0.05)。在正常对照组、黄芪干预组和肝纤维化模型组大鼠血清中TGF-β1表达含量分别为491.29pg/L、629.91pg/L、959.09pg/L,呈递增趋势。模型组与前两组比较差异均有显著性(P<0.01),黄芪干预组与正常组比较其表达含量无显著差异(P>0.05)。结论黄芪注射液可以显著抑制实验性肝纤维化大鼠模型的肝纤维化,这种抑制作用可能与抑制I型胶原蛋白及TGF-β1的表达有关。

Objective To explore the therapeutic effect of astragalus injection on liver fibrosis, type Ⅰ collagen （Col- Ⅰ ） and TGF- β1 was measured by immunochemistry and enzyme linked immunosorbent assay（ELISA） in a rat model. Methods Thirty-five SD rats were randomly divided into normal control group, carbon tetrachloride （CCl4）-induced liver fibrosis model group and astragalus injection group. CCl4 had been injected sub- cutaneously for ten weeks,after CCl4 intervention, all rats were killed immediately. Immunohistochemistry was used to measure the expression of Col- Ⅰ in liver tissue. The level of TGF- β1 in serum was measured by ELISA. Results The CCl4-induced rat liver fibrosis model was established suc- cessfully. The expression of Col- Ⅰ in liver tissues and the level of TGF- β1 in serum increased significantly with the development of liver fibrosis. The average OD values of Col-Ⅰ were 0.298,0.315 and 0.503 in the normal control group, astragalus injection group and fibrosis group, respectively. The difference was significant between fibrosis group and the other groups （P 〈 0.01）, while the difference was not significant between normal control group and astragalus injection group（P 〉 0.05）. The level of TGF-β1 were 491.285pg/L,629.9 10pg/L,959.086pg/L in the normal control group, astragalus injection group and fibrosis group, respectively. The difference was significant between fibrosis group and the other groups （P 〈 0.01）, while the difference was not significant between normal control group and astragalus injection group（P 〉 0.05）. Conclusion Astragalus injection could significantly inhibit liver fibrosis by reduces the expression of Col- Ⅰ and TGF-β1 in the experimental rat model of liver cirrhosis.