白术挥发油提取方法改进

New Extraction Method of Volatile Oil of Atractylodes Macrocephala Koidz

目的:改进《中国药典》(下称"药典")中白术挥发油的提取方法。方法:按药典规定的挥发油提取测定方法提取测定白术挥发油,同条件下按本改进方法提取测定白术挥发油;用气/质联用法分别分离鉴定2种挥发油成分。结果:按药典方法提取测定的白术挥发油收率为(1.28±0.03)%(n=6),同条件下按本试验改进方法收率为(1.39±0.03)%(n=6)。此外,与本方法相比,药典法提取时有7种白术挥发油被漏掉。结论:改进的植物挥发油提取方法增加了挥发油的收率和种类,明显优于药典方法。

OBJECTIVE： To improve the method of Chinese Pharmacopoeia on the extraction of volatile oil from Atractylodes rnacrocephala Koidz..METHODS： Volatile oil was extracted from Atractylodes rnacrocephala Koidz. and deter- mined by the extraction method and determination method stated in Chinese Pharmacopoeia and by the new improved method respectively. And the components of volatile oil obtained in these two methods were respectively separated and identified by GC-MS. RESULTS： The extraction rates of volatile oil by the method of Chinese Pharmacopoeia vs. the new method were （1.28±0.03）% （n = 6） vs. （1.39±0.03） % （n = 6）.As compared with the new method, seven components were missed by the method of Chinese Pharmacopoeia. CONCLUSION： The new extraction method of volatile oil can increase both the extraction rate of volatile oil and the kinds of the components in volatile oil, and it is obviously better than the method stated in Chinese Pharmacopoeia.