谷胱甘肽(GSH)高产酵母菌株的初筛及营养条件优化

Initial Screening of High-yield Yeast Strain and Optimization of Nutritional Conditions for Glutathione(GSH)

对实验室保藏的14株不同种属的酵母菌株的谷胱甘肽(GSH)产量进行了比较,发现其中log酵母菌株的GSH产量最高,达127.785 mg/L。对其营养条件进行了研究,发现其最佳碳源为葡萄糖;硝酸盐会抑制其生长,而有机氮源对其生长有利;考虑到磷和钾对酵母细胞生长及发酵代谢的作用,选择KH_2PO_4为培养基中的基本成分。采用正交试验对培养基的基本成分进行优化,在最佳培养条件下log酵母的GSH产量有较大幅度的提高,达到148.547 mg/L。

Comparing the yield of glutathione from 14 different species of yeast strains kept in the Key Lab of Hubei Industrial Microbiology,it was found that the highest glutathione yield was 127.785 mg/L achieved by Saccharomycodes ludwigii log.The nutritional conditions were further investigated.Results showed that the optimum carbon source was glucose.Nitrate could inhibit the growth of yeasts,and organic nitrogen sources could benefit the growth.For the growth of yeast cell and the metabolism in fermentation,KH2PO4 was selected as one of the basic ingredients of culture medium.The basic medium ingredients were also optimized with orthogonal test.The yield of GSH by Saccharomycodes ludwigii log could be greatly improved and up to 148.547 mg/L.