摘要
目的探讨中药白花蛇舌草抑制人宫颈癌Hela细胞肿瘤活性的作用及分子生物学机制。方法MTT法检测白花蛇舌草对宫颈癌Hela细胞生长的影响,采用倒置显微镜观察细胞形态学变化,化学比色法测定Hela细胞活性氧(ROS)产生状态,流式细胞仪检测细胞凋亡率,免疫组化染色并用计算机图像分析观察p53基因表达。结果白花蛇舌草可抑制Hela细胞的生长,其效果与药物的浓度和作用的时间相关,倒置显微镜下Hela细胞出现细胞凋亡的形态学改变,流式细胞仪检测到凋亡峰,白花蛇舌草作用后细胞内ROS水平下降,而突变型p53表达降低。结论一定浓度的白花蛇舌草对人宫颈癌Hela细胞具有抑制增殖和诱导凋亡的作用,其机制可能与细胞内ROS水平和p53基因调控有关。
Objective To study the anti -tumor effect of Hedyotic diffusa （HD） on the Hela cell line and analyze its molecular biological mechanisms. Methods The inhibitory effect of HD on cell growth was analyzed by MTT assay; the changes in apoptosis, by flow cytometry; the production of reactive oxygen species （ROS）, by chemical colorimetry; the cell morphologic changes, by HE staining; the protein expression of p53, by immunocytochemical staining. Results Hedyotic diffusa could inhibit the proliferation of Hela cells in a time - and dose - depend manner, with the rate of apoptosis increased with the concentration of drug. The intracellular content of ROS and the expression of p53 were decreased significantly. Conclusion HD can induce the apoptosis of Hela cells, and its molecular mechanism may be associated with the down - regulation of ROS and p53 expression.
出处
《徐州医学院学报》
CAS
2007年第9期571-574,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省医药管理局课题(H-054)
