摘要
桑天牛卵啮小蜂(Aprostocetus prolixus)有孤雌生殖现象,其后代几乎为雄性。沃尔巴克氏体(Wolbachia)是广泛分布于节肢动物体内的一类共生菌,参与多种调控寄主的生殖活动机制。通过对外膜蛋白基因(wsp)、细菌细胞分裂蛋白基因(ftsZ)和核糖体16S rDNA基因的特异性扩增,在米蛾(Corcyra cephalonica Stainton)的DNA中分别扩增出约600、1000和900 bp的片断,验证了米蛾体内已感染了Wolbachia;而在桑天牛卵啮小蜂的DNA中未扩增出任何片断,表明Wolbachia在桑天牛卵啮小蜂体内未被感染或感染率极低。
Aprostocetus prolixus has a phenomenon of parthenogenesis and its offspring are almost male. Wolbachia is a common and widespread group of bacteria found in arthropods. These bacteria evolve various mechanisms for manipulating reproduction of their hosts. Based on the amplification of the wsp, ftsZand 16S rDNA gene fragments of 600 bp, 1 000 bp and 900 bp in length, respectively from the DNA of a laboratory population of Corcyra cephalonica Stainton, it is proved that the Corcyra cephalonica Stainton has been infected with the Wolbachia;But no any fragment from the DNA of the Aprostocetus prolixus was amplified, suggesting that the Aprostocetus prolixus was not infected by Wolbachia or the infection rate was extremely low.
出处
《蚕业科学》
CAS
CSCD
北大核心
2007年第3期448-451,共4页
ACTA SERICOLOGICA SINICA
基金
河北省林业局科技项目(编号0534302)
