维胃方对实验性大鼠胃溃疡血清NO和NOS的影响

Effect of Weifeifang on NO and NOS of Blood Serum in Experimental Rat with Gastric Ulcer

目的:观察维胃方对实验性大鼠胃溃疡血清一氧化氮(NO)和一氧化氮合酶(NOS)的影响,探讨维胃方修复胃黏膜损伤的可能机制。方法:采用冰醋酸烧灼法,建立胃溃疡模型。以PH试纸检测胃酸变化,用公式S=π.d1/2.d2/2计算溃疡面积,比色法测定血清NO和NOS含量,并观察胃黏膜组织病理学变化。结果:与模型组比较,维胃方能明显修复腺泡,促进胃酸分泌,恢复胃液PH值(P<0.01),其中高、中剂量组接近正常组水平;维胃方高、中剂量组与模型组比较可显著降低大鼠血清中NO含量(P<0.01),抑制iNOS活性(P<0.01),增强cNOS活力(P<0.05);维胃方能不同程度减小胃黏膜损伤(P<0.01),其中以维胃方中剂量效果最优,与雷尼替丁组存在着显著性差异(P<0.05),高、低剂量组与雷尼替丁组无差异性(P>0.05);自愈组溃疡面积与模型组无显著性差异(P>0.05)。结论:维胃方对实验性胃溃疡具有明显的保护作用,其机制可能是通过降低大鼠血清中异常升高的NO水平和iNOS活性,进而减轻炎症介质对胃黏膜的损伤。

Objective： To investigate the effect of Weiweifang on NO and NOS of blood serum in experimental rats with gastric ulcer and to probe the possible mechanism of Weiweifang repairing stomach mucosa damage. Methods： The model rats were induced by icy acetic acid, and the secretion of gastric acid was detected by PH paper. Gastric mucosa ulcer area was calculated by the formula of S = π·d1/2· d2/2. Colorimetric method was used to determine the content of NO and NOS in blood serum. Meantime, the pathologic change of gastric mucosa was examined. Results ： Comparison of results between model group and experimental group found that Weiweifang could recover gland alveolus obviously, enhance the secretion of gastric acid, and raise the PH value of gastric juice （ P 〈0.01）. The experimental results of Weiweifang with high and middle dosage approximated thai of the nomal group. Comparison also found that Weiweifang with high and middle dose could lower the contents of NO in blool serum （ P 〈 0.01 ）, inhibit iNOS activity （ P 〈 0.01 ）, and reinforee eNOS activity （ P 〈 0.05 ）. The results also showed that Weiweifang could alleviate mucosa damage （ P 〈 0.01）, with the middle dose functioning best. In the function of mueosa damage alleviation, there was significant difference between Weiweifang with middle dose group and ranitidine group （ P 〈 0.05）, and no special discrepancy between Weiweifang with high and low dose group and ranitidine group （ P 〉 0.05）. Between self-cure group and model group, there was no discrepancy in terms of ulcer area. Conclusion： Weiweifang can exert a significant positive effect against experimental gastric ulcer, possibly by decreasing NO level and NOS activity and easing gastric mucosa damage of inflammation mediators.