禁用兽药氯霉素人工抗体的制备及特异吸附性

Preparation and Characteristics of Artificial Antibody for the Banned Antibiotic-Chloramphenicol

为建立氯霉素分子印迹吸附检测方法,开发氯霉素新型检测试剂盒,本文对氯霉素人工抗体的制备及其吸附性质进行了研究.采用分子印迹技术优化确立了人工抗体的制备方法.结果表明,以氯霉素为模板分子,4-乙烯吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,四氢呋喃为致孔剂,模板分子、功能单体、交联剂比例为1∶1∶10,所制备的氯霉素人工抗体对氯霉素的特异性吸附达54.42μmol/g,可在10 min内快速达到吸附平衡,磺胺噻唑和磺胺二甲吡啶对氯霉素人工抗体的交叉反应小,分别为9.71%和6.24%.因此,将所制备的人工抗体应用于食品中氯霉素残留的快速检测具有相当的优势.

In order to establish the molecularly imprinted adsorption assay of chloramphenicol, the preparation and adsorption of chloramphenicol was studied in this paper. Using chloramphenicol as the template molecule and 4-vinylpyridine as the functional monomer, the bulk polymer was prepared in the optimal condition. Equilibrium binding experiments was investigated to evaluate the binding characteristics of the chloramphenicol artificial antibody and increase understanding of the mechanisms of recognition in molecularly imprinted polymers. Analysis showed that the maximum binding capacity was estimated to be 54.42 μmol/g, the adsorption process reached equilibrium within 10 minutes. The selectivity of artificial antibody was evaluated by cross reactivity of chloramphenicol and other compounds （ sulfathiazole and sulfamethazine ） , the results showed that the imprinted polymer exhibited high affinity for chloramphenicol among the tested compounds, that make it suitable for the detecting of chloramphenicol residue in foods.