四翅滨藜无性系组培快繁技术研究

Rapid Multiplying Technique by Clone Tissue Culture in Atriplex Canescens

用四翅滨藜茎段、茎尖、嫩叶等外植体在不同培养基上培养,结果表明:茎段、茎尖外植体的诱导芽和愈伤组织诱导率高,启动诱导培养基以1/2 MS+KT 0.5 mg/L+2,4-D 1.0 mg/L最好;诱导愈伤组织增殖的培养基以MS+KT 1.0 mg/L+2,4-D 0.05 mg/L最好,增殖率可达3倍以上;丛生芽增殖培养基以MS+KT 0.5 mg/L+NAA 0.1 mg/L和MS+6-BA 0.5 mg/L+NAA 0.1 mg/L效果好,增殖率均在5倍以上;生根培养基以1/4 MS+NAA 0.5 mg/L和1/4 MS+IBA 1.0 mg/L效果好。组培快繁取材广泛,繁殖系数高,是加快四翅滨藜种苗繁育的有效途径。

External plant parts such as stem parts, stem tips and tender leaves of Atriplex canescens were Cultured on different media, the result shows that high induced budding and high induced rate of healing tissue from stem parts and stem tips happened. Initiating culture medium of induced budding is optimum based on 1/2 MS ＋ KT 0.5 mg/L ＋ 2,4 - D 1.0 mg/L; The culture medium inducing healing tissue multiplication is optimum based on MS ＋ KT 1.0 mg/L ＋ 2,4 - D 0.05 mg/L, in which ,multiplying rate reached as high as three times ;The culture medium of cespitose budding multiplication is optimum based on MS ＋ KT 0.5 mg/L ＋ NAA 0.1 mg/L and MS ＋ 6 - BA 0.5 mg/L ＋ NAA 0. 1 mg/L, in which, multiplying rate averagely reached more than five times;The rooted culture medium is optimum based on 1/4 MS ＋ NAA 0.5 mg/L and 1/4 MS ＋ IBA 1.0 mg/L;Rapid multiplying technique of tissue culture is an effective way to accelerate multiplication for plantlet of Atriplex canescens,with extensive material and high multiplying coefficiency.