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MAPREC技术在OPV质控中应用的研究进展 被引量:3

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摘要 本文介绍了用聚合酶链反应(PCR)和限制性内切酶酶切技术作突变分析(MAPREC)的方法和原理,及其在口服脊髓灰质炎疫苗(OPV)质控中的应用.
出处 《国外医学(预防.诊断.治疗用生物制品分册)》 1997年第1期3-7,共5页 Foreign Medical Sciences(Section of Bilogics for Prophylaxis,Diagnosis and Therapy)
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  • 1郭仁,中国医学科学院学报,1998年,20卷,70页
  • 2彭小忠,国外医学.预防、诊断、治疗用生物制品分册,1997年,20卷,1期,3页
  • 3梁晓峰,罗凤基,封多佳,译.疫苗学[M].北京:人民卫生出版社,2011:104-105.
  • 4WHO.Poliomyelitis Fact sheet,No.114[OL].(2014-10-xx)[2014-11-11].http://www.who.int/mediacentre/factsheets/fs114/zh/.WHO.世界卫生组织脊髓灰质炎实况报道,第114号[OL].http://www.who.int/mediacentre/factsheets/fs114/zh/.
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  • 6Gnanashanmugam D,Falkovitz-Halpern MS,Dodge A,et al.Shedding and reversion of oral polio vaccine type 3 in Mexican vaccinees:comparison of mutant analysis by PCR and enzyme cleavage to a real-time PCR assay[J].J Clin Microbiol,2007,45(8):2419-2425.
  • 7Liang XF,Luo FJ,Feng DJ,et al.Vaccines[M].Beijing:People′s Medical Publishing House,2011:690-702.
  • 8Skinner MA,Racaniello VR,Dunn G,et al.New model for the secondary structure of the 5′non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence[J].J Mol Biol,1989,207(2):379-392.
  • 9Cann AJ,Stanway G,Hughes PJ,et al.Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine[J].Nucleic Acids Res,1984,12(20):7787-7792.
  • 10Minor PD.The molecular biology of poliovaccines[J].J Gen Virol,1992,73(Pt 12):3065-3077.

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