原发性肝癌中GSTM1基因多态与p53 249 密码子突变的相关研究

ASSOCIATION BETWEEN GSTM1 GENE POLYMORPHISM OF PRIMARY HEPATOCELLULAR CARCINOMA AND MUTATION OF p53 CODON 249

为了探索肝癌的发生是否同人体解毒酶系统中某些解毒酶、如ＧＳＴ的功能缺陷有关，我们检测了１４７个正常个体和４５例原发性肝癌癌组织ｐ５３基因２４９密码子突变情况。结果发现ＧＳＴＭ２－ｎｕｌ等位基因在正常人群中的频率为０．７１，而在患者中为０．８２，经统计检验其差异有显著性。在３７个原发性肝癌患者中，发现２４９密码子突变８例，且均为ＧＳＴＭ１－ｎｕｌ纯合子。表明ＧＳＴＭ１在肝癌的演化和发生中，经解毒黄曲霉毒素而起重要作用。

The GSTM1 gene polymorphism of both the peripheral white cells of 147 normal subjects and the normal liver tissue of 45 patients with primary hepatocellular carcinoma(PHC), and the mutation of p53 codon 249 of liver cancer tissue of 37 patients with PHC were detected. It was found that the allele frequency of GSTM1 null type among general population was 0.71, while that among PHC patients was 0.82. The difference between the two allele frequncies was statistically significant. Of the 37 PHC patients detected, 8 were found positive in codon mutation; in addition, all of these 8 cases were homozygotes of GSTM1 null type.