GA_3对于芹菜种子休眠打破及TDZ对芹菜分化的影响

The Effect of Gibberellin(GA3)on the Dormancy of Celery (Apium graveolens L.)and the Effect of TDZ on the Proliferation of Celery

以芹菜为研究材料,研究了萌发,生长,分化,生根,培养条件等因素对组培苗的生长影响。结果表明:收获1年的芹菜种子,用10 mg/L GA3浸泡12 h后,以0.1%HgCl2消毒90 s,再铺种于含10 mg/L GA3的MS中,6 d后萌发,在20℃下14 d后萌发率可达到70%以上,28℃时萌发率明显降低;以MS为基本培养基;1.0 mg/L BA+1.0 mg/L TDZ使其分化率达到100%,30 d后平分化数达到5个;单独使用TDZ时,1.0 mg/L TDZ的综合效果最好;0.01mg/L NAA下根的诱导率是100%,14 d后平均生根数达到7.2。培养条件以20℃为宜。

This study discussed the effect of Gibberellin（GA3） on dormant celery seeds, and TDZ on proliferation of celery.The result showed that celery dipped in 10 mg/L GA3 and plated on MS with 10 mg/L GA3 performed better（more than 70 % in 20 ℃ ） than these with no disposal on the germination. Temperature is another factor during germination, and in high temperature（ 〉 25 ℃ ）, the germination rate were restrained. Choose MS medium as the basic medium, with 1.0 mg/L BA ＋ 1.0 mg/L TDZ,the differentiation rate reached 100%, and the shoot number achieved 5. With 0.01 mg/L NAA, the induce rate of roots achieved 100 %, and the average roots per seedling reached 7.2.