大白鼠海马CA_1区锥体细胞的急性分离及其电生理学特性

ACUTE ISOLATION AND ELECTROPHYSIOLOGICAL PROPERTIES OF CA_1 PYRAMIDAL NEURONS IN HIPPOCAMPUS OF RATS

目的：建立适用于膜片组技术（Patchclamptechnigue，PCT）的大白鼠海马CA_4区锥体细胞（rathippocampalCApyramidalneuron，RHCPN）的急性分离方法，并测定该细胞的电生理学特性。方法：用7～21d大鼠，以链霉蛋白酶E酶解加吸管吹打法制备RHCPN，用全细胞PCT测定其电生理学特性。结果：分离的RHCPN易用于PCT，且具有正常的电生理学特性，并保存了主要的电压依赖性和受体门控性高于通道特性。结论：本法分离的RHCPN适用于PCT电生理学研究。

To acutely isolate rat hippocampal CA1 pyramidal neurons (RHCPN) suitable forpatch clamp technique (PCT), and to measure their electrophysiological properties. Methods:RHCPNwere obtained by pronase E treatment and then by trituration with pipette from 7 to 21 day-old rats.PCT was used to study their electrophysiological properties. Results:The isolated RHCPN were easy torecord by PCT. Their electrical and chemical membrane preperties concerning some major voltage -de-pendent and receptor-gated ionic channels were well-preserved. ConcIusion: The RHCPN isolated bythis method is suitable for PCT electrophysiological study.