慢性低氧大鼠肺动脉内皮素mRNA的表达及定位

ENDOTHELIN mRNA EXPRESSION AND ITS LOCALIZATION IN INTRAPULMONARY ARTERIES OF CHRONIC HYPOXIC RATS

目的：探讨慢性低氧大鼠肺动脉内皮素－1基因表达及分布。方法：采用生物素标记cRNA探针，对Wistar大鼠肺动脉进行原位杂交。结果：低氧1周组及低氧2周组大鼠大部分肺动脉内皮细胞ET－1mRNA表达呈阳性信号，而对照组大鼠仅极少数阳性信号（P＜0．01）；低氧1周组大鼠部分显示强阳性信号。低氧1周组及低氧2周组大鼠部分肺动脉平滑肌细胞呈现ET-1mRNA表达阳性信号，而对照组大鼠则无阳性信号表达（P＜0．01及0．05）。结论：慢性低氧对大鼠肺动脉ET－1分泌的影响是在基因转录水平进行，且主要细胞定位在肺动脉内皮细胞，此外还包括肺动脉平滑肌细胞。

To study endothelin-1 (ET-1) gene expression and its distribution in intrapulmonary arteries of chronically hypoxic rats. Methods: in situ hybridization studies were performed onpulmonary arteries in Wistar rats using biotinlabelled cRNA probe for ET-1. Results: Most of intrapulmonary arteries showed ET-1 mRNA positive expression (+) in endothelial cells of either one-week ortwo-week hypoxic rats, whereas only few intrapulmonary arteries did in those of control rats (P＜0.01). Furthermore, some intrapulmonary arteries expressed intense ET-1 mRNA signals (+ +) in endothelial cells. ET-1 was expressed (+) in pulmonary artery smooth muscle cells of either one-week ortwoweek hypoxic rats but no signal was observed in those of controls (P ＜ 0．01 and 0. 05,respectively). Conclusion: Chronic hypoxia alters ET-1 gene transcription mainly in the pulmonaryartery endothelial cells and then smooth muscle cells.