卡巴胆碱改善重度烫伤大鼠胃血流量和胃排空功能的初步研究

Investigation of the effect of carbachol on improving gastric emptying and gastric blood flow in rats with severe scald injury

目的:探讨不同剂量卡巴胆碱对烫伤大鼠胃血流量和胃排空功能的影响。方法:40只Wistar雄性大鼠,随机分为假烫组(n=5)、单烫组(n=5)和口服卡巴胆碱组(n=30),后者又根据卡巴胆碱剂量不同分为20、40、60、80、100、120μg/kg组(每组5只)。假烫组大鼠均给予0·1%酚红溶液1·5ml灌胃。单烫组和口服卡巴胆碱组大鼠均经30% TBSAⅢ度烫伤后立即给予0·1%酚红溶液1·5ml灌胃,口服卡巴胆碱组于伤后0·5h给予相应剂量卡巴胆碱+1ml生理盐水灌胃。大鼠均于伤后2h处死,观察不同剂量卡巴胆碱对大鼠胃血流量及胃排空率的影响。结果:烫伤后大鼠胃排空率为(29·50±13·42)%,明显低于假烫组(92·19±5·24)%,P<0·01。伤后2h胃血流量从伤前的(476·00±71·24)U降低到(103·60±51·28)U,P<0·01。烫伤后给予不同剂量卡巴胆碱均能增加胃排空率和胃血流量,但以60μg/kg最为显著(P<0·01)。结论:卡巴胆碱对烧伤休克大鼠胃血流量和胃排空有显著促进作用,最佳剂量为60μg/kg。

Objective：To investigate the effects of different doses of carbachol on gastric emptying and gastric blood flow in rats with scald injury. Methods：Forty Wistar male rats were randomly divided into sham-scald group, scald injury group and scald injury＋different doses of carbachol groups（20 μg/kg, 40 μg/kg, 60 μg/kg, 80 μg/ kg, 100 μg/kg, 120 μg/kg respectively gavaged） of carbachol respectively（each n = 5）. 1.5 ml of phenol red was gavaged to each rat in sham-scald group. Same amount of phenol red was gavaged in other two groups after 30% total body surface area （TBSA） full thickness scald injury. Carbachol was garaged with an oral tube about 30 minutes after scald injury according to the weight of individual rat. All rats were sacrificed 2 hours after injury to calculate the gastric emptying ratio and determine the gastric blood flow. Result： The gastric emptying ratio in scald injury group was （29. 50±13. 42）% ,and it was lower than gastric emptying ratio in sham-scald group（92. 19±5. 24） % （P〈0.01）. The gastric blood flow was decreased from （476.00±71.24）U in sham-scald group to （103.60 ±51.28） U in scald injury 2 hours after injury（P〈0.01）. All doses of carbachol had the effect of improving gastric emptying ratio and gastric blood flow, but the most effective dose was 60μg/kg[gastric emptying ratio： （73. 08± 20.82） % ; gastric blood flow： （ 188.20 ± 14.41 ） U]. Conclusion： Carbachol can apparently promote the gastric blood flow and the gastric emptying with 60μg/kg as the best dosage.