木樨草素拮抗庆大霉素耳毒性实验观察

Experimental study on luteolin rivalizes ototoxicity induced by gentamicin

目的:初步了解木樨草素对庆大霉素耳毒性的影响。方法:设庆大霉素组与庆大霉素加不同剂量木樨草素(10mg、20 mg)两组,并进行组间比较,观察听性脑干反应(ABR)阈值、眼震电图(ENG)的频率,了解其听功能及前庭功能的变化。结合耳蜗基底膜铺片琥珀酸脱氢酶(SDH)染色方法观察耳蜗形态学变化。结果:听功能检查结果显示,庆大霉素加不同剂量木樨草素组的ABR阈值明显低于庆大霉素组,经统计学处理,差异有显著性。形态学检查结果与听功能检查结果一致。前庭功能检查结果亦发现木樨草素对庆大霉素引起的前庭功能变化有拮抗作用。结论:本实验发现木樨草素对庆大霉素引起的耳蜗和前庭损伤有拮抗作用。

Objective：To primarily find out the effect about luteolin rivalizing ototoxicity of gentamicin.Methods： In this experimental study,gentamicin group was compared to the group of gentamicin with different dose of luteolin （10 mg,20 mg）.The changes in hearing and vestibule function were discovered by measuring the threshold of ABR and the frequency of ENG.The method of cochlear preparation stained with SDH was used to observe morphological changes in cochleas.Results： The auditory examination results showed that the thresholds of the group of gentamicin with different dose of luteolin were higher than the one of the gentamicin group,and the difference was significant in statistics.The morphology analysis result was consistent with auditory examination. And the vestibule function examination also proved that luteolin rivalize vestibule function changes induced by gentamicin.Conclusion： Based on the experiment,luteolin rivalize ototoxicity induced by gentamicin.