摘要
用硫酸铵分级沉淀，离子交换纤维素柱层析分离技术，对香菇〔Ｌｅｎｔｉｎｕｌａ（Ｌｅｎｔｉｎｕｓ）ｅ－ｄｏｄｅｓ〕子实体漆酶进行分离纯化，得到单一组分的漆酶，通过ＳＤＳ－ＰＡＧＥ凝胶电泳法，测得其摩尔式量为５５０００。研究表明该酶的最适ｐＨ为２．６，最适温度为７０℃，酶的动力学研究测出米氏常数Ｋｍ值为０．１４２ｍｍｏｌ。
Through systematical process for isolation and purification of laccase from fresh fruit body of Lenlinula edodes using the technique (NH 4) 2SO 4 fractionation and ionexchange chromatography.We obtained one component of laccase,it showed homogeneity in the gel electrophorosis.The molecular weight was 55 000 by SDS PAGE,The optimum pH, temparature were 2.6 ℃ and 70 ℃,respectively. The studies of enzyme kinetics showed K m =0 142 mmol
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
1997年第2期50-53,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
关键词
生漆
香菇
子实体
漆酶
纯化
米氏常数
Lentinula edodes
Fruiting body
Laccase
Purification
Michaelis constant
