系统性红斑狼疮患者外周血调节性T细胞与IL-10及TGF-β_1的检测

Detection of CD4^+CD25^+ Regulation T Cells and Related Cytokine in Peripheral Blood in SLE Patients

目的:研究系统性红斑狼疮(SLE)患者外周血CD4+CD25+调节性T细胞(regulation T cells,Tr细胞)及相关细胞因子的变化,分析它们在SLE发病机制中的作用。方法:收集SLE患者及健康对照组外周抗凝静脉血,分离纯化T淋巴细胞。PE标记的抗CD4单抗,FITC标记的抗CD25单抗,作双色流式细胞术,分析SLE患者外周血CD4+CD25+调节性T细胞百分率,ELISA法检测调节性T细胞相关细胞因子的表达。结果:SLE患者外周血Tr细胞的数量明显低于正常对照组[(2.83±1.05)%vs(5.07±0.59)%,P<0.05];患者血清中IL-10、TGF-β1的含量均低于正常对照组,差异有显著性[IL-10:(29.48±13.69)pg/mlvs(43.10±14.95)pg/ml,P<0.05;TGF-β1:(170.04±91.58)pg/mlvs(254.75±130.41)pg/ml,P<0.05]。患者外周血Tr细胞的比例与血清中IL-10、TGF-β1的含量呈正相关(r1=0.53,r2=0.64,P<0.05)。结论:SLE患者外周血存在细胞免疫功能失调,CD4+CD25+调节性T细胞数量与功能状态发生改变,T淋巴细胞耐受机制的破坏可能与SLE的免疫学发病机制有关。

Objective To investigate the amount of CD4^＋CD25^＋ regulation T cells and the level of related cytokine in peripheral blood in SLE patients, and analyze the relationships between CD4^＋CD25^＋ regulation T cells and the immunopathogenesis of SLE. Methods CD4^＋CD25^＋ regulation T cells were detected by flow cytometry in peripheral blood. Besides, related cytokines in peripheral blood were assayed by ELISA. Relativity between CD4^＋CD25^＋ regulation T cells and related cytokine was analyzed. Results CD4^＋CD25^＋ regulation T cells were decreased obviously in peripheral blood in SLE patients compared with control group [ （2.83 ± 1.05） % vs（5.07 ±0.59） % ,P 〈0.05]. The level of related cytokine was also decreased in SLE groups compared with control groups [ IL-10 ： （ 29.48 ± 13.69 ） pg/ml vs （ 43.10 ± 14.95 ） pg/ml, P 〈 0.05 ; TGF-β1 ： （ 170.04 ± 91.58 ） pg/ml vs （ 254.75 ± 130.41 ） pg/ml, P 〈 0.05 ]. There were positive relationships between CD4^＋ CD25^＋ regulation T cells and related cytokine IL-10, TGF-β in SLE patients （ r1 = 0.53, r2 = 0.64, P 〈 0.05 ）. Conclusion Celluar immunological function disorder is observed in SLE patients, and the abnormal expression of CD4^＋ CD25^＋ T cell and related cytokines in peripheral blood may involve in SLE immunopathogenesis.