慢性应激对大鼠葡萄糖耐量及胰岛素抵抗的影响

Effects of chronic stress on glucose tolerance and insulin resistance in rats

目的:探讨慢性应激对大鼠葡萄糖耐量及胰岛素抵抗的影响。方法:将40只SD大鼠按体重随机分成应激组和对照组各20只。应激组通过8周的慢性复合应激刺激作为慢性应激动物模型,对照组在此期间正常饲养。在试验4、8周监测大鼠体重、旷场实验、24h尿皮质醇,在实验8周采尾血检测葡萄糖耐量(酶氧化法)、空腹胰岛素(化学发光法),并计算胰岛素抵抗和B细胞功能。结果:慢性应激大鼠4、8周后体重下降显著(P<0.05),有明显的行为学改变。应激组大鼠OGTT曲线高峰前移,下降缓慢,在0、30min时两组血糖差异显著(P<0.05)。应激组大鼠空腹胰岛素、30min胰岛素、胰岛素抵抗、B细胞功能以及24h尿皮质醇与对照组之间差异显著(P<0.01)。结论:慢性应激引起大鼠糖耐量异常,胰岛素抵抗增加。

Objective： To Explore the effects of chronic stress on glucose metabolism in rats. Methods： Forty male Sprague-Dawley rats were randomly divided into stress and control groups according to body weight, with twenty in each. The different weight, open-field experience, OGTT, insulin resistance and B cell function in rats in different groups were investigated. Results： Compared with the control group, the body weight, open-field experience, gJucose tolerance, insulin, insulin resistance, B cell function and urinary cortisol level of the stress group were significantly differente. Conclusion： The study suggests that chronic mild stress contribute to abnormal glucose tolerance and induce insulin resistance.