盐胁迫下外源ABA对谷子耐盐愈伤组织生理生化特性的影响

Changes of Physiological and Biochemical Characteristics in the Original and NaCl-Tolerant Calli of Setaria italica Exposed to Abscisic Acid and Sodium Chloride

外源ABA可使谷子胚性愈伤组织生长减缓，使正常胚性愈伤组织在NaCl胁迫下与耐盐胚性愈伤组织的生长差异消失，脯氨酸含量在无NaCl或1％NaCl胁迫下分别提高140％和9．3％，而可溶蛋白含量均下降，并有新的SDS电泳蛋白质带（90KD）出现，过氧化物酶活性及SOD活性也均增高。

Some physiological and biochemical chances in the original and NaCItOlerant embryogenic calli of Setaria italica cultured on the medium containing ABA 10μmol/L and different levels of NaCl were studied. The difference in callus growth between original and NaCl-tolerant calli disappeared under ABA treatment though the calli growth rate was decreased (Fig. 1 ). The following chances in the original calli were oh served after being treated with ABA:1. On NaCl-free and 1 %NaCl-containing media the proline content was increased by 140% and 9. 3% respechvely (Fig. 2); 2. The contents of soluble proteins were decreased in both NaCl-stressed and unstressed calli (Fig.3 ). Their electrophoresis patterns changed, and a new band of 90 kD protein appeared(Fig. 4); 3. aam achvihes of peroxidase and superoxide dismutase (AN) were increased by 54. 4% and 65. 1% respechvely by ABA in NaCl-free medium (Table 1, Fig. 6 ). The chances caused by ABA in NaCl-tolerant callus were similar to those occurring in the original callus.