昆明山海棠在微核实验中非整倍体毒性的研究

The Study on the Aneuploidy Induced byTripterygium hypoglaucum (Level) Hutch in Micronucleus Test

本文报道以小鼠着丝粒次要卫星ＤＮＡ探针ＦＩＳＨ和抗着丝粒ＣＲＥＳＴ染色，研究了可疑的非整倍体毒剂昆明山海棠（ＴＨＨ）诱导的小鼠ＮＩＨ３Ｔ３细胞微核（ＭＮ）的着丝粒组成情况，结果ＴＨＨ（１０～６０μｇ／ｍｌ）诱导的６２１～６８４％的ＭＮ为ＦＩＳＨ阳性，３５９～４２２％的ＭＮ为ＣＲＥＳＴ阳性，较精确的ＦＩＳＨ结果显示ＴＨＨ具有较强的非整倍体诱发效应，同时认为次要卫星ＤＮＡ探针比ＣＲＥＳＴ染色更适合于ＭＮ的着丝粒检测。

The fluorescence in situ hybridization (FISH) with minor satellite DNA probe and antikinetchore antibodies (CREST staining) techniques were applied to study the kinetochore compositions of micronuclei(MN)induced by a suspect aneugen Tripterygium hypoglaucum (Level) Hutch (THH) in mouse NIH3T3 fibroblast cell lines. The results showed the labelling frequencies of MN with the FISH and CREST were identical 621～ 684% and 369～422% respectively. The precise results of FISH indicated that THH has stronger aneuploidy toxicity. Comparision of the results between the CREST staining and FISH, the minor satellite DNA probe is more suitable to detect the kinetochores in MN.