摘要
利用RAPD分子标记对山杨、欧洲山杨、银白杨、响叶杨及毛白杨的5个不同类型,即抱头毛白杨、截叶毛白杨、易县毛白杨、陕西毛白杨和南京栽培的毛白杨进行了分析,并进行了银白杨×响叶杨正反交实验,研究杂种后代与毛白杨的关系。RAPD分析说明,毛白杨的亲缘关系与银白杨和响叶杨较近,而与山杨和欧洲山杨的亲缘关系则较远。毛白杨在聚类图上首先是与银白杨和响叶杨聚为一类,再与山杨和欧洲山杨聚在一起。通过比较毛白杨与银白杨和响叶杨的正反交子代,发现毛白杨更接近银白杨×响叶杨的子代,而与反交子代有所区别。由此可见,毛白杨为天然杂种是没有疑义的,而且很可能是银白杨×响叶杨的杂种。本研究筛选出的13个引物,产生了78条谱带,覆盖了基因组的一定区域,其可靠性是较高的。
This study analyzed five varieties of P. tomentosa and their putative parents, P. alba, P. davidiana, P. adenopoda and P. tremula by RAPD and artificial cross. Of 78 bands revealed by 13 arbitrary primers selected, 58(74.36%) bands were polymorphic among P. tomentosa and four putative parents. The RAPD statistical results showed the relationship between P. tomentosa and P. alba or P. adenopoda was closer than that between P. tomentosa and'P. davidiana or P. tremula . There were higher similarity and lower genetic distance between P. tomentosa and P. alba or P. adenopoda than that between P. tomentosa and the other two putative parents. The results from UPGMA, Fitch-margoliash phylogenetic dendrogram were similar. In addition, artificial hybrids including reciprocal cross between P. alba and P. adenopoda were used to compare with five varieties of P. tomentosa . And those hybrids between P. alba as female and P. adenopoda as male were closer to P. tomentosa than those from reciprocal cross. The results demonstrated that P. tomentosa was a natural hybrid between P. alba as female parent and P. adenopoda as male parent.
出处
《植物分类学报》
CSCD
1997年第1期24-31,共8页
Acta Phytotaxonomica Sinica
基金
林业部重点课题资助
关键词
毛白杨
DNA多态性
起源
RAPD
Populus tomentosa
DNA polymorphismas
Origin
Phylogenetic dendrogram
