摘要
本文用原位杂交技术动态观察了慢性低氧大鼠肺内原癌基因jun、fos和myb的表达,结果发现:(1)正常大鼠肺内有一定量的junmRNA表达,少见到myb及fos的表达;(2)低氧1周时,jun的表达较正常下降,2周后有所增加。低氧3周后,jun的mRNA表达较正常明显上升;(3)低氧1、2周后,myb的表达明显增加,3周时基本恢复到正常水平;(4)fos原癌基因在低氧1、2周时有一定量的表达,低氧3周时达最大值。提示低氧可刺激大鼠肺内原癌基因jun、myb和fos的表达。
This paper is to investigate the expression of oncogene jun、fos and myb mRNA in the lung of rats exposed to chronic hypoxia.15 SD rats were put in low oxygen chamber (FiO 2=0.1),8hrs daily for 1,2 and 3 weeks.Five rats breathing room air served as control.Oncogene expression in lung tissue assessed by the use of in situ hybridization.The results showed that (1) there was a slight expression of jun mRNA but not fos and myb mRNA in the control normoxic rats' lung; (2) it was found that a less expression of jun mRNA in lung after 1 week hypoxia, but after 2 week hypoxia jun mRNA elevated again and significantly increased after 3 week hypoxia as compared with that in normoxia; (3) the oncogene myb mRNA expression showed significant increase in 1 and 2 week hypoxia and returned to normal status in 3 week hypoxia; (4) after 1 to 3 week hypoxia, a significant increased expression of fos mRNA was found as compared with that in normoxia.It is suggested hypoxia may induce increased expression of proto oncogene jun、myb and fos ,which may be related to proliferation of pulmonary arterial smooth muscle cells and fibroblasts.
出处
《中国应用生理学杂志》
CAS
CSCD
1997年第1期21-24,共4页
Chinese Journal of Applied Physiology
关键词
肺动脉高压
慢性
低氧
原癌基因
chronic hypoxia
lung
oncogene
in situ hybridization