慢性低氧对大鼠肺内几种原癌基因表达的影响

EFFECTS OF CHRONIC HYPOXIA ON THE EXPRESSION OF ONCOGENE jun、fos AND myb mRNA IN RAT LUNG

本文用原位杂交技术动态观察了慢性低氧大鼠肺内原癌基因ｊｕｎ、ｆｏｓ和ｍｙｂ的表达，结果发现：（１）正常大鼠肺内有一定量的ｊｕｎｍＲＮＡ表达，少见到ｍｙｂ及ｆｏｓ的表达；（２）低氧１周时，ｊｕｎ的表达较正常下降，２周后有所增加。低氧３周后，ｊｕｎ的ｍＲＮＡ表达较正常明显上升；（３）低氧１、２周后，ｍｙｂ的表达明显增加，３周时基本恢复到正常水平；（４）ｆｏｓ原癌基因在低氧１、２周时有一定量的表达，低氧３周时达最大值。提示低氧可刺激大鼠肺内原癌基因ｊｕｎ、ｍｙｂ和ｆｏｓ的表达。

This paper is to investigate the expression of oncogene jun、fos and myb mRNA in the lung of rats exposed to chronic hypoxia.15 SD rats were put in low oxygen chamber (FiO 2=0.1),8hrs daily for 1,2 and 3 weeks.Five rats breathing room air served as control.Oncogene expression in lung tissue assessed by the use of in situ hybridization.The results showed that (1) there was a slight expression of jun mRNA but not fos and myb mRNA in the control normoxic rats' lung; (2) it was found that a less expression of jun mRNA in lung after 1 week hypoxia, but after 2 week hypoxia jun mRNA elevated again and significantly increased after 3 week hypoxia as compared with that in normoxia; (3) the oncogene myb mRNA expression showed significant increase in 1 and 2 week hypoxia and returned to normal status in 3 week hypoxia; (4) after 1 to 3 week hypoxia, a significant increased expression of fos mRNA was found as compared with that in normoxia.It is suggested hypoxia may induce increased expression of proto oncogene jun、myb and fos ,which may be related to proliferation of pulmonary arterial smooth muscle cells and fibroblasts.