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禽腺病毒细胞病变观察和半数组织感染量测定 被引量:3

Observation of Cytopathogenic Effect Caused by Avian Adenovirus and Determination of TCID_50.
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摘要 从贵州省的患病鸡群中分离出一株禽腺病毒,暂定名为HS—1。HS一1毒株能够在鸭胚成纤维细胞上生长良好,第1代即出现明显的细胞病变,细胞培养上清液中病毒的血凝效价达到2 ̄(13)。采用交叉血凝抑制试验证实,贵州分离毒HS-1与标准毒株AV—127属于同一血清型或相关血清型,其半数组织感染量经测定达到10 ̄(-3.58)/0. An avian adenovirus was isolated from affected flock in Guizhou province, designated as HS-1. HS-1 stain grew well at duck embryo fibroblast, the cytopathogenic effect could be observed at the first passage and the virus hemoagglutination titer reached 2^(13) in the supernatant of cell culture. It was demonstrated by crossing hemoagglutination inhibitory test that Guizhou isolate HS-1 and standard strain AV127 belonged to the same serotype or the related serotype. The TCID_(50) of HS-1 strain was determined as 10^(-3.56)/0.1 ml.
出处 《贵州农学院学报》 1997年第1期17-21,共5页
关键词 禽腺病毒 腺病毒 细胞病变 半数组织感染量 鸡病 avian adenovirus cytopathogenic effeet serotype TCID_(50)
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