镉对小鼠睾丸生殖细胞DNA损伤作用研究

Effects of Cadmium on DNA Damage of Mice Testicle Germ Cell in vitro

目的研究氯化镉对小鼠睾丸生殖细胞DNA的损伤作用。方法用不同浓度氯化镉分别对小鼠睾丸细胞进行处理,分为阴性对照组(生理盐水)、微剂量组(0.04mmol\LCdCI2)、低剂量组(0.2mmol\L CdCI2)、中剂量组(1mmol\LCdCI2)、高剂量组(5mmol\L CdCI2),用单细胞凝胶电泳法分别计数各组400个细胞,按照不同的损伤程度进行分类;并分别测量各组50个彗星细胞尾长并计算均方值。结果各组小鼠睾丸生殖细胞损伤率和尾长均方分别为:阴性对照组:8.0%、8.543±2.131,微剂量组:48.5%、24.761±3.595,低剂量组:65.0%、39.246±5.894,中剂量组:71.5%、42.745±5.231,高剂量组:87.5%、49.374±4.543;4个剂量组小鼠睾丸细胞DNA损伤率显著高于阴性对照组(P<0.001),四个剂量组小鼠睾丸细胞DNA损伤的彗星细胞尾长均方值显著高于阴性对照组(P<0.001),并且DNA损伤率和彗星细胞尾长均方值随着剂量增加而增加。结论微量氯化镉对小鼠睾丸生殖细胞DNA即已存在明确损伤作用,且损伤程度与氯化镉剂量成正比。

Objective To study the effect of cadmium chloride on DNA damage of mice testicle germ cell in vitro. Methods Isolated mice testicle germ cell were exposed to cadmium chloride with the concentrations of 0, 0.04, 0.20, 1.0, 1.5 and 2.0 mmol, respectively, for one hour. The proportion of DNA damage and the length of DNA migrations were determined by single cell gel electrophoresis （SCGE）. Results The DNA damage rate and the length of DNA migrations of isolated mice testicle germ cell on cadmium chloride were higher than that of normal group （p 〈 0.001 ）. Results also showed the obvious dose - response relationship between the DNA damage （ the rate of DNA damage and the length of DNA migrations） and doses of cadmium chloride. Conclusions Cadmium chloride can induce DNA damage on isolated mice testicle germ cell at eertain dose.The DNA damage on testicle germ cell is more impressible.