运脾散对厌食症动物模型大鼠血浆脑肠肽的影响

The regulating effect of Yunpi Powder on Brain-Gut peptide in plasma of anorexia rats

目的探讨运脾散预防和治疗小儿厌食症的机理。方法病因模拟法制作幼龄厌食症大鼠模型,经运脾散治疗后,用放射免疫检测技术测定外周血浆八肽胆囊收缩素(CCK-8)和β-内啡肽(β-EP)的含量。结果与空白组比较,模型大鼠血浆CCK-8含量均显著增高(P<0.05),血浆β-EP含量显著减低(P<0.01);与模型组比较,治疗组血浆CCK-8含量显著降低(P<0.05),血浆β-EP含量显著增加(P<0.05)。结论运脾散能在一定程度上防治小儿厌食症,与调节该模型外周血浆β-EP和CCK-8的分泌与释放有关。

Objective To explore the mechanism that Yunpi Powder prevent and cure symptom of wean anorexia. Methods To observe the effect of Yunpi Powder on Brain-Gut peptide concentration in plasma in juvenile rat model of anorexia. The β-EP and CCK-8 concentration in plasma were examined by radioimmunoassay. Results Compared with control rats, the CCK-8 concentration in plasma in the model rats increased, while the β-EP concentration decreased. Compared with model rats, the CCK-8 concentration normalize decreased and β-EP increased significantly in treatment group. Conclusion Yunpi Powder can improve appetite and weight in the anorexia rats models. And the possible reason is that they can regulate the secretion and release of β-EP and CCK-8 in peripheral areas.