SV40灭活疫苗免疫恒河猴后的抗体反应

Antibody Response Induced by Inactivated SV40 Vaccine in Macaques

目的 评价SV40灭活疫苗免疫恒河猴后的抗体反应.方法 用SV40灭活疫苗经肌肉注射免疫0.5～1周岁的恒河猴,分别于第1次和第2次免疫后2周,检测血清中和抗体效价,免疫18个月后检测SV40基因组成分.结果 免疫的12只恒河猴在第1次免疫后2周,血清抗体全部阳转,平均抗体滴度为1：16,第2次免疫2周后血清抗体滴度明显增长,平均达到1：64～1：128.用PCR方法未检测到SV40基因组成分.结论 SV40灭活疫苗可以有效诱导恒河猴产生特异性抗体反应.

Objective To evaluate the antibody response induced by inactivated SV40 vaccine in macaques. Methods Immunize macaques aged 6 - 12 months by intramuscular injection with inactivated SV40 vaccine for 2 times, at an interval of 10 weeks. Collect the venous blood 2 months after the 1st and the 2nd immunization respectively for determination of serum neutralizing antibody titer,and the peripheral blood 18 months after the 2nd immunization for SV40 genomic component. Results The serum antibody positive conversion rate of all the 12 macaques 2 weeks after the 1st immunization was 100% ,and the mean antibody titer was 1： 16. However,the mean antibody titer 2 weeks after the 2nd immunization reached 1：64 - 1： 128. No SV40 genomic component was detected by PCR in the peripheral blood of macaques 18 months after the 2nd immunization. Conclusion Inactivated SV40 vaccine induced specific antibody response in macaques.