在无血清无蛋白培养基中培养Vero细胞

Culture of Vero Cells in Serum-free and Protein-free Medium

目的开发在无血清无蛋白培养基(PF)中培养Vero细胞的技术。方法在DMEM/F12培养基中添加酵母提取物和大豆水解物,配制成无血清无蛋白培养基。使用配制好的无血清无蛋白培养基,分别在玻璃、聚乙烯醇缩丁醛(PVB)和聚苯乙烯(PS-TC)培养面上培养Vero细胞,观察细胞生长情况,并绘制细胞生长曲线,以含血清培养基BM为对照。结果与BM培养基比较,在玻璃培养面上,PF培养基不支持Vero细胞生长和分裂;在PVB培养面上,PF培养基支持Vero细胞生长,但不支持分裂;在PS-TC培养面上,PF培养基支持Vero细胞的生长和分裂。结论在PS-TC培养面上,PF培养基适合培养Vero细胞。

Objective To develop a method for euhure of Vero cells in serum-free and protein-free medium. Methods Prepare serum-free and protein-free medium（PF） by adding yeast extract and soy hydrolysate into DMEM/F12 medium. Culture Vero cells in the prepared medium in common glass, polystyrene （PS-TC）- and polyvinyl butyrul （PVB）-coated glass flasks respectively, using serum-containing medium （BM） as control. Observe the growth of Vero cells and plot a growth curve. Results Compared with BM, in common glass flask, the prepared PF medium could not support the growth and division of Vero cells; in PVB-eoated glass flask, the medium supported the growth but could not support the the division of Vero cells. However, in PS-TC flask, the prepared PF medium supported both growth and division of Vero cells. Conclusion The prepared serum-free and protein-free medium was suitable for the euhure of Vero cells in PS-TC flask.