摘要
采用2亲本杂交的方法将Tn5-luxAB标记基因系统成功地转入了分离自小麦根圈的PGPR菌株荧光假单胞菌(Pseudomonasfluorescens)Xl6菌株中。与亲本菌株相比,携有发光酶基因luxAB的标记菌株Xl6L2生理生化特征的变化很小,而且仍保留有在小麦根圈的定殖能力。Tn5-luxAB片段在Xl6L2中的稳定性很强,在没有卡那霉素选择压力的情况下,luxAB也能稳定表达。即使在40℃温度下。
Tn5 luxAB gene marker system was sucessfully transfered into a wheat PGPR strain Pseudomonas fluorescens Xl6 with biparental mating. Changes of physiological and biochemcal characteristics of luxAB marked strain Xl6L2 were few compared with its parent strain. Xl6L2 remained good capability of wheat root colonization. Tn5 luxAB genes in Xl6L2 were very stable. Even if there was no Km selective pressure, and could express luciferase activity. Xl6L2 could also emit light at 40℃. It can be used in studying molecular ecology of PGPR in wheat rhizosphere extensively.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1997年第3期220-225,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金
国际科学基金